MouseembryoE16fulllengthcDNAismadeofRNAextractedfromwholeembryousingclassicalguanidineisothiocyanate-phenol:chloroformextractionmethod. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA.ThecDNAisprimedwitholigodTprimer.

QualityControl:TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.

ThesynthesizedcDNAisalsousedastemplateforPCRamplificationofß-actingene.PCRproductofß-actinwasvisualizedon1%agarosegel.

Applications:cDNAisidealforgeneexpressionanalysisbyPCR,characterizationofalternativesplicingofmRNA,verificationofgeneticmutation,genecloningandtargetsequencing.

Packing/shipping:eachcDNAsampleisroutinelyshippedondryicein1.5mlvialsandisenoughfor30reactions(30PCRamplifications).

MSDSandCertificateofAnalysisinPDFfiles:ContactZyagenTechnicalSupportatzinfo@zyagen.com